Indonesia Conference Directory

Corresponding Author
Marham Sitorus

Institutions
a) Department of Chemistry, Universitas Negeri Medan, Medan 20221, Indonesia
*marham.sitorus[at]gmail.com

Abstract
Malondialdehyde (MDA) is a metabolite resulting from lipid peroxidation by free radicals that can be formed when hydroxyl free radicals such as Reactive Oxygen Species (ROS) react with fatty acid components of the cell membrane so that a chain reaction is known as fat peroxidation. The fat peroxidation will break the chain of fatty acids into various toxic compounds and cause damage to the cell membrane. Thus MDA is an indicator of the presence of free radicals in the body. In this research, the Conjugated Linoleic Acid (CLA) antioxidant test was synthesized from castor oil in vivo against Wistar rats. Wistar rats were exposed to free radicals through cigarette smoke for 2 hours per day for 14 days. Mice were given an antioxidant intake of CLA with a concentration of 200, 400, 600,800,1000 mg / body weight every day with three repetitions. The same thing is done with intake of Ascorbic Acid (Vitamin C) and &

Keywords
MDA; CLA; Free Radicals and Inhibition

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/tdnJ7E64PKuv

Corresponding Author
RIRIN DWI ASTUTI

Institutions
1) Biology Education of Postgraduate School Universitas Negeri Medan, Jalan Willem Iskandar Psr V Medan Estate, North Sumatra 20221, Indonesia.
2) Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Medan, Jalan Willem Iskandar Psr V Medan Estate, North Sumatra 20221, Indonesia.
*fauziyahharahap[at]gmail.com

Abstract
This research was conducted to determine the best combination of plant growth regulators on callus induction of shoot explants mangosteen (Garcinia mangostana L.). The research used complete randomized design (CRD) factorial which is consisting of 12 treatments with 3 times repetitions. The parameters observed were time of callus formation, callus texture, callus color, callus pile height, callus surface area, callus weight, and callus performance. Data was analyzed by using ANOVA, and DMRT as follow up. The result showed that addition of plant growth regulators have effect on callus induction of shoot explant mangosteen (Garcinia mangostana L.). The best concentration and composition of media for induction of mangosteen callus is by giving sucrose 1,5 g / L + 2,4D 3 ppm because it was able to produce callus with the fastest time with a compact texture and color that is identical to green and white. The callus has 0,67a cm in height, 1,70a cm2 in width, and 0,063a g in weight. The callus performance is growing all over the surface.

Keywords
callus induction, in vitro, mangosteen, plant growth regulator.

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/E4LndNqzQApB

Corresponding Author
Murniaty Simorangkir

Institutions
a) Department of Chemistry, Universitas Negeri Medan, Medan 20221, Indonesia
*murniatysimorangkir[at]unimed.ac.id

Abstract
Sarang banua plants are found in Simalungun, North Sumatra, Indonesia, which have been used by the community as traditional medicinal plants and is a type of Clerodendrum fragrans Vent Willd, including the family Verbenaceae. The study design was a completely randomized design (CRD) with five treatments namely 1% Na-CMC solution (K1, negative control), bovine serum antigen (K2), ethanol extract of sarang banua leaf 200 mg/kg BW (K3), ethanol extract of sarang banua leaf 200 mg/Kg BW with bovine serum antigen (K4) and ethanol extract of sarang banua leaf 150 mg/Kg BW with bovine serum antigen (K5). The results showed that administration of ethanol extract of sarang banua leaf (Clerodendrum fragrans Vent Willd) significantly affected serum globulin levels of rabbits (Oryctolagus cuniculus). The ethanol extract of sarang banua leaves 200 mg/kg BW with bovine serum antigen (K4) produced the highest serum globulin levels of rabbits (12.91 mg/mL).

Keywords
Sarang banua (Clerodenrum fragrans Vent Willd); leaf ethanol extract; globulin Rabbit serum

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/RZEqjVBfdrzK

Corresponding Author
Tita Juwitaningsih

Institutions
a) Department of Chemistry, Universitas Negeri Medan, Medan 20221, Indonesia
* juwitaningsih[at]gmail.com

Abstract

Keywords
North Sumatera Cardamom Seed (Amomum compactum); Antibacterial; Breast anticancer (MCF-7)

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/HRjEFNVPJW4K

Corresponding Author
Fera Kurniadewi

Institutions
Chemistry Study Program, Universitas Negeri Jakarta, Jalan Rawamangun Muka, Jakarta 13220
*fera[at]unj.ac.id

Abstract
The Isolation and Purification of ethyl acetate fraction from Muntingia calabura leaves have resulted three flavonoid derivatives. UV-Vis, 1H-NMR dan 13C-NMR data showed that isolate compounds are 2,4-dihydroxychalcone (1), 3,7-dimetoxy-5-hydroxyflavon (2) and 3,5,7-trihydroxy-8-methoxyflavone (3). The antioxidant evaluation against 2,2-diphenyl-1-picrylhydrazyl (DPPH), compound (3), showed to be a very high activity (IC50 3,7 x109 ppm). The isolated compounds were also evaluated for their cytotoxic activities against murine leukemia P-388 cells resulted that all isolated compounds were inactive.

Keywords
Antioxidant, cytotoxic activity, flavonoid, Muntingia calabura

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/jkN4dqKrYQPC

Corresponding Author
Tumiur Gultom

Institutions
Lecturer of Biology Department, Mathematics and Natural Science Faculty, Universitas Negeri Medan Medan, North Sumatera, Indonesia
Graduated of Biology Department, Mathematics and Natural Science Faculty , Universitas Negeri Medan, Medan, North Sumatera, Indonesia
Researcher ,Research Center for Vegetables Crops,Tongkoh, Berastagi , North Sumatera, Indonesia

Abstract
This study aimed to determine the growth and production of Doulu Cultivar Garlic of MV2 generation. This research was conducted in March-July 2019. The seeds used in this study came from MV1 Garlic which given Gamma Rays at dose of 6 Gy (G3), 8 Gy (G4) dan 10 Gy (G5). Planting seeds in carried out in a research center for vegetables crops, Tongkoh, Berastagi, North Sumatera. The design used in this research is Non Factorial Randomized Block Design with 4 replications. This research uses 4 levels of treatment in accordance with the source of the seeds the dose 0 Gy (G0), 6 Gy (G3), 8 Gy (G4) dan 10 Gy (G5). The seeds came from 67 tubers consisting of 340 cloves. The parameters observed are age of growth, height of plant, number of leaves, number of stomata, diameter of stem, weight of cloves, diameter of cloves, and number of cloves. Data from this study were analysed with Anova and Least Significant Different test (LSD test). The results showed that the growth and production of plants significant of effect based on seed by radiation doses that are on height of plants, number of leaves, diameter of stem, weight of bulbs, diameter of bulbs, weight of cloves, diameter of cloves, structure of bulbs, and shape of bulbs of cultivar Doulu garlic but no significant on age of growth, number of stomata, and number of cloves.

Keywords
garlic, gamma ray radiation, MV2 generation

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/eRuHC6DqGTfQ

Corresponding Author
mislah sahila harahap

Institutions
(1 )Biology Education of Postgraduate school Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V Medan Estate, North Sumatera 20221, Indonesia
(2)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V, North Sumatera 20221, Indonesia *fauziyahharahap[at]gmail.com
(3)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Jalan Bioteknologi No.1, North Sumatera 20155, Indonesia

Abstract
Orchids are plants that has aesthetic value and high economic important. Included in to ornamental plant whose existence is diminishing and endangered. Therefore, to preserve this plant it is necessary to plant one way through in vitro techniques. The aim of this study was to determine the best interaction of growth regulator Benzyl amino purine, coconut water and the interaction of both multiplication of Dendrobium sp. The research method used a completely randome design with various concentrations of Benzyl amino purine (BAP; 0,1,2,3 mg/L) and coconut water concentrations (0%,5%,10%). Were the observation parameter are number of shoots, leaves and roots. Data were analyzed descriptively and inferentially with ANOVA. The results shows the real effect of BAP interaction, between coconut water shoots and leaves, but did not significantly affect the number of roots. Within 12 weeks of observation the highest number of shoots and leaf obtained from a combination of 10% coconut water + BAP 3 mg/L were 12.00 and 5.00. The lowest number of shoots and leaves was obtained in combination of 5% coconut water + BAP 1 mg/L, 6.00 and 1.00.

Keywords
Benzyl amino purine, Coconut water, Dendrobium sp., In vitro

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/HvYTtdXD9jzR

Corresponding Author
YULIANA FERNANDO

Institutions
(1)Biology Education of Postgraduate School Universitas Negeri Medan, Jalan Willem Iskandar Pasar V Medan Estate, North Sumatera 20221, Indonesia.
(2)Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Medan, Jalan Willem Iskandar Pasar V Medan Estate, North Sumatera 20221, Indonesia.
*fauziyahharahap[at]gmail.com
(3)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera utara, Jalan Bioteknology No.1, North Sumatera 20155, Indonesia

Abstract
Pineapple is a plant that needs to be developed on a plantation scale because the fruit has economic important. The research was conducted to determine the best media combination on the growth of pineapple from sipahutar In Vitro. The research used complete randomized design (CRD) factorial which is consisting of 12 treatments with 3 times repetitions. The parameters observed were number of shoots, leaves and roots. Data was analyzed by using ANOVA, and DMRT as follow up. The results showed addition of BA and vitamins have effect on propagation of pineapple shoots from sipahutar. The highest number of shoots was 8.00 shoots and 19.00 leaves produced MS + Vit 2 ppm + BA 2 ppm media. The highest root number of 3.67 produced MS + Vit 0 ppm + BA 0 ppm media.

Keywords
Benzyl Adenin, in vitro, Pineapple,Vitamin

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/Kg2vwjbADTJ8

Corresponding Author
Saronom Silaban

Institutions
(a) Department of Chemistry, Universitas Negeri Medan, Medan 20221, Indonesia
*silabans[at]gmail.com

Abstract
Amylase is a starch hydrolyzing enzyme. This protein is very important for various industrial processes. Microorganisms are considered as the best source of amylase production. This study aims to isolate amylolytic bacteria from rice soil samples that have the potential to produce amylase enzymes. Paddy soil samples were collected from two locations, Percut Sei Tuan and Lubuk Pakam rice field. The isolates obtained were then characterized macroscopically and microscopically. Macroscopic characterization was carried out by observing the morphology of bacteria with a microscope while microscopic characterization was carried out by the Catalase Test, Endospora Staining Test and Gram Staining. From the isolation that has been done, 14 isolates have the potential to produce amylase enzymes. The obtained isolates were tested with Iodine staining. Isolates that had been macroscopically characterized showed results such as: irregular shape, slippery edges, branched, wavy, circular, having macroscopic characterization. raised and convex colony elevation, white and beige. The isolates which were characterized by the catalase test showed positive results for all isolates. Characterization with Endospora Staining showed 7 isolates positive while 7 isolates negative. For the characterization by gram staining, obtained 7 isolates were gram positive and 7 isolates were gram negative. Thus, from the two sample locations obtained amylolytic bacteria that potentially produce amylase enzymes.

Keywords
amylase; bacteria; isolation; rice soil

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/43bqze6uZvXB

Corresponding Author
FAUZIYAH HARAHAP

Institutions
1Department of Biology, Faculty of Mathematics and Natural Sciences, Medan State University, Jl. Williem Iskandar Pasar V Medan Estate, Medan, 20221, Indonesia
2 Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatera, Jalan Biotechnology No.1, USU Campus Medan, 20155, Indonesia
3Department of Agronomy and Horticulture, Faculty of Agriculture, Agriculture University, Bogor, Indonesia.

Abstract
Pineapple (Ananas comosus L.) from Sipahutar is an herbaceous plant from Sipahutar District. Pineapple canning industry sourced from Sipahutar pineapple has the opportunity to be developed in North Sumatra. Propagation through in vitro culture is an alternative method to solve this problem. The purpose of this research was to determine the regeneration ability of pineapple callus derived from callus induction with the addition of 2,4-D 1 ppm and BAP 1 ppm. The callus were regenerated using the addition of Kinetin (0; 0.5; 1; 1.5 ppm). Each treatment was carried out 4 times. The results showed: 1) the fastest time of emergence of shoots (5 days) came from 0.5 ppm, 2) formed the most shoots (8 shoots) resulting from the addition 0 ppm and 0.5 ppm, 3) the most number of leaves (7.75 leaves) resulted from light treatment with the addition kinetin 0.5 ppm and 1 ppm, 4) the longest leaf length (4.44 cm) from dark treatment with kinetin 0.5 ppm, 5) the highest plant height (5.32 cm) from dark treatment with 0.5 ppm kinetin. This shows that the concentration of 0.5 ppm kinetin with the dark treatment is the best concentration to stimulate the formation of pineapple shoots.

Keywords
Pineapple, kinetin, regeneration, in vitro

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/8ZbRrwVtAPXQ

Corresponding Author
MUKHTAR ISKANDAR PINEM

Institutions
UNIVERSITY OF SUMATERA UTARA

Abstract
Potato (Solanum tuberosum L.) in Indonesia is a commodity that priorities to developed, and has the potential to be marketed in the domestic as well as export . One of the obstacles is important that faced is pathogen soil-borne which is caused by the Ralstonia solanacearum. This pathogen can causing loss of yield of potato 40-100%. Bacillus sp. and P. fluorescens are antagonistic microbes that are proven to be able to control bacterial wilt disease (R. solanacearum) by 69,95 % and 61,67%. This study aimed to determine the effect of the comparison composition of growing media and the ability of some agents of biological within suppress the growth of the disease wilt bacteria of potatoes which is caused by R. solanacearum . This research using completely randomized design (CRD) nonfaktorial with 10 treatments, namely: M0(top soil), M1(top soil, R. solanacearum), M2(top soil, R. solanacearum, P. fluorescens), M3(top soil, R. solanacearum, Bacillus subtilis), M4 until M9 are combination of them. The results showed that the application of biological agents was able to suppress the growth of the pathogen R. solanacearum, as well as significantly affect plant height, number of shoots, and production.

Keywords
potato, R. solaanacearum, P. fluorescens, B. subtilis

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/qDRbfA4eVwky

Corresponding Author
MIFTAH ARINA HARAHAP

Institutions
1) Biology Education of Postgraduate School Universitas Negeri Medan, Jalan Willem Iskandar Psr V Medan Estate, North Sumatra 20221, Indonesia.
2) Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Medan, Jalan Willem Iskandar Psr V Medan Estate, North Sumatra 20221, Indonesia.
*fauziyahharahap[at]gmail.com

Abstract
This research was conducted to find out the effect of ab mix nutrient on growth and yield of Pak choi (Brassica chinensis L.) plants under hydroponic wick system condition. The experiment was conducted in non factorial completely randomized design (CRD), 3 replications of five levels concentration of ab mix (0, 650, 1300, 1950, 2600 ppm). Each replication consist of 6 plants, therefore there were 90 plants observed. The parameters observed were plants height, number of leaves and width, fresh and dry weight, harvest index, and root volume. Data was analyzed by using one way ANOVA, and DMRT as follow up. The result showed effect of ab mix on 1) plants height, 2) number of leaves 3) leaf width 4) fresh weight 5) dry weight 6) harvest index, but none on root volume. The best ab mix concentration for parameters of number of leaves (18.78 leaves), leaf width (47.32cm2), fresh weight (33.57gr), dry weight (2.40gr) and harvest index (99.15percent) was in 1950 ppm. The best ab mix concentration for parameters of plants height (8.71cm) was in 2600 ppm.

Keywords
ab mix, hydroponic, nutrient, pak choi, wick system

Topic
Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/6e2AvrDxqmHN